Search results for "shedding vesicle"

Extracellular membrane vesicles can mediate intercellular transfer of molecules

2012

Many eukaryotic cell types, including neural cells, release into the extracellular environment vesicles of different sizes and composition. Neurons and astrocytes shed extracellular vesicles which contain FGF2 and VEGF and could be involved in interaction with endothelial cells, to form the blood-brain barrier. Also brain tumor cells, such as glioblastomas, release vesicles in the extracellular space. Microvesicles (MVs) shed from G26/24 oligodendro¬glioma cells were previously reported to contain FAS-L and to cause a reproducible, dose-dependent, inhibitory effect on neurite outgrowth, and neuronal apoptosis, when added to primary cultures of rat cortical neurons. More recently, they were …

Extracellulare membrane vesicles as vehicles for cell-to-cell and cell-to-extracellular matrix functional interactions

2012

Co-culture of rat brain cells as a tool for studying cell-cell interactions

2012

Brain capillary endothelial cells (BCECs) form the blood-brain barrier (BBB) in response to interaction with other brain cells (astrocytes, pericytes and neurons). BCECs are characterized by tight junctions (TJ), maturation and stabilization of which require different proteins, such as occludin. When co-cultured with astrocytes and neurons, BCECs were found to form a monolayer resembling the natural BBB: paracellular flux of dopamine and sucrose (i.e. compounds which are unable to cross the BBB in vivo) significantly decreased (1), while the transendothelial electrical resistance (TEER) increased. In these conditions, BCECs produced a larger amount of occludin and tended to localize it at t…